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для подбора праи� меров к этим консервативным участкам использовали программу primerselect пакета lasergene  (DNASTAR)
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Fig. 1. Electropherogram of the PCR results for the rye GT47 gene fragment: M – 1 kb molecular weight marker (Evrogen, Russia); 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’
для подбора праи� меров к этим консервативным участкам использовали программу Primerselect пакета Lasergene, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 1. Electropherogram of the PCR results for the rye GT47 gene fragment: M – 1 kb molecular weight marker (Evrogen, Russia); 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’
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Image Search Results


Fig. 1. Electropherogram of the PCR results for the rye GT47 gene fragment: M – 1 kb molecular weight marker (Evrogen, Russia); 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’

Journal: Proceedings on applied botany, genetics and breeding

Article Title: Analysis of nucleotide sequences of the <i>GT47</i> glycosyltransferase gene in rye cultivars differing in the content of water-soluble pentosans in grain

doi: 10.30901/2227-8834-2023-2-112-119

Figure Lengend Snippet: Fig. 1. Electropherogram of the PCR results for the rye GT47 gene fragment: M – 1 kb molecular weight marker (Evrogen, Russia); 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’

Article Snippet: Для подбора праи� меров к этим консервативным участкам использовали программу PrimerSelect пакета Lasergene (DNAStar, США), некоторые термодинамические параметры праи� меров оценивали с помощью онлаи� н-утилиты Oligoanalyzer, специфичность проверяли с помощью BLAST (https://blast.ncbi.nlm.nih.gov).

Techniques: Molecular Weight, Marker

Fig. 2. Alignment results for the GT47 gene fragments in the studied rye cultivars: 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’ (nucleotide numbering was made according to the consensus sequence of the GT47 gene for all analyzed cultivars, formed with the MegAlign program)

Journal: Proceedings on applied botany, genetics and breeding

Article Title: Analysis of nucleotide sequences of the <i>GT47</i> glycosyltransferase gene in rye cultivars differing in the content of water-soluble pentosans in grain

doi: 10.30901/2227-8834-2023-2-112-119

Figure Lengend Snippet: Fig. 2. Alignment results for the GT47 gene fragments in the studied rye cultivars: 1 – ‘Chulpan 7’; 2 – ‘Podarok’; 3 – ‘KVS Aviator’; 4 – ‘KVS Magnifico’; 5 – ‘KVS Eterno’ (nucleotide numbering was made according to the consensus sequence of the GT47 gene for all analyzed cultivars, formed with the MegAlign program)

Article Snippet: Для подбора праи� меров к этим консервативным участкам использовали программу PrimerSelect пакета Lasergene (DNAStar, США), некоторые термодинамические параметры праи� меров оценивали с помощью онлаи� н-утилиты Oligoanalyzer, специфичность проверяли с помощью BLAST (https://blast.ncbi.nlm.nih.gov).

Techniques: Sequencing